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Individual Cells Isolated From Biological Clock Can Keep Daily Time, But Are Unreliable

September 10, 2009

Alexis Webb enters a small room at Washington University in St. Louis with walls, floor and ceiling painted dark green, shuts the door, turns off the lights and bends over a microscope in a black box draped with black cloth. Through the microscope, she can see a single nerve cell on a glass cover slip glowing dimly.

The glow tells her the isolated nerve cell is busy keeping time.

Webb, a graduate student in the neuroscience program, working with Erik Herzog, Ph.D., associate professor of biology in Arts & Sciences; Nikhil Angelo, an undergraduate biology major; and James Huettner, Ph.D., associate professor of cell biology and physiology in the School of Medicine, has demonstrated that individual cells isolated from the biological clock can keep daily time all by themselves.

However, by themselves, they are unreliable. The neurons get out of synch and capriciously quit or start oscillating again.

The biological clock, a one-square millimeter area of the brain called the suprachiasmic nucleus, or SCN, just above the roof of the mouth and atop the crossing of the optic nerves, comprises about 20,000 neurons.

These cells, remarkably, contain the machinery to generate daily, or circadian, rhythms in gene expression and electrical activity. But the individual cells are sloppy and must communicate with one another to establish a coherent 24-hour rhythm, says Herzog.

These features make the SCN a flexible clock that can reset to stay in synch in an ever-changing environment. The underlying sloppiness is probably what allows us to adjust to local time when we cross time zones and to vary our sleep cycles with the season, say the WUSTL researchers.

The research is being published the week of Sept. 7 in the online Early Edition of the Proceedings of the National Academy of Sciences.

“We’ve known for more than 15 years that unicellular organisms like cyanobacteria can keep 24-hour time, and isolated cells from the marine snail eye can as well,” says Herzog. “But nobody was sure whether individual cells in vertebrates are circadian pacemakers.”

The SCN includes many kinds of neurons that make different neurochemicals and connections within the SCN and to other parts of the brain.

“Some scientists felt that all of the cells in the SCN would be intrinsically rhythmic and that there was nothing special about any of them,” says Herzog. “Some thought that none of the cells would be rhythmic and that the rhythm arose instead from their network interactions, and a third group thought specialized SCN neurons would be rhythmic and the others wouldn’t be at all capable. Our experiments proved all three hypotheses wrong.”

Capturing the rhythm

Webb digested slices of mouse SCN with enzymes to isolate individual neurons and then plated the cells sparsely on a dish. “The neurons will actually attach to the glass and grow,” says Webb. “And as long as you give them all of the nutrients they need, they’ll live for months.”

The cells had been genetically engineered to glow whenever they expressed the time-keeping gene Period 2. (The cells came from transgenic mice where the Period 2 gene had been linked to one found in firefly tails.)

The rhythmically waxing and waning glow was detected by a camera designed to capture the light from distant stars and so sensitive that it will register the passage of even a single cosmic ray.

The recordings showed that all cells seem to be able to keep a 24-hour rhythm — there are no special pacemaker cells — but they don’t seem to do it all the time. Neurons that make different neurochemicals show circadian rhythms in gene expression, and none was more dependable than the others.

“Single cells sometimes will be very robust and rhythmic, but most of the time they quit or lose the rhythm,” says Webb. “It appears that the network structure of the SCN is important for stabilizing these sloppy intrinsic rhythms.”

To show that different kinds of SCN neurons did not have rigidly defined roles, Webb exposed SCN to the drug TTX, a pufferfish toxin that shuts down cell-to-cell communication. “In a sense we just isolated the nerve cells again,” she says, “but chemically rather than physically and in a reversible way.”

She washed off the TTX, and then added it again, to see if the second time the cells were exposed to the toxin, they would behave the same way.

“We found cells that changed their behavior,” she says. “So the first time they were isolated, or uncoupled, with TTX, they continued to oscillate, but the second time they stopped oscillating. But we also saw the reverse: cells that were non-oscillatory becoming oscillatory.”

Paradoxically the sloppiness of the clock is what makes it so precise.

“The SCN is the master clock that synchronizes other biological clocks, like your liver or your lung. Those peripheral clocks can keep 24-hour time, but not for very long,” says Herzog. “Because the SCN is built differently, it can self-sustain — it can keep on ticking like a good Timex.”

The researchers are now focusing on the connections that help synchronize and stabilize these biological oscillators.

Comments

64 Responses to “Individual Cells Isolated From Biological Clock Can Keep Daily Time, But Are Unreliable”

  1. Brandon Archer - 5th period on September 10th, 2009 9:09 pm

    Alex Webb the first person to see a single nerve cell glowing on a glass cover slip. He believes that the cell is keeping time. He graduated in the nuero science program with Erik Herzog. The cells are practically biological clocks. They arent that reliable and start and stop over time.

  2. Crystal Whitehouse --- 6th Period on September 11th, 2009 4:35 pm

    ~ Alexis Webb sees a glow when bending over a microscope in a black box draped with black cloth
    ~ The glows tells her the islolated nerve cell is bus keeping time
    ~ The biological clock just above the roof of the mouth and atop the crossing of the optic nerves, comprises about 20,000 neurons
    ~ These cells contain the machinery to generate daily or circadian rhythms in gene expression and electrical activity
    ~ But the individual cells are sloppy and must communicate with one another to establish a coherent 24-hour rhythm; these features make the SCN a flexible clock that can reset to stay in synch in an ever-changing environment
    ~ Webb exposed SCN to the drug TTX, a pufferfish toxin that shuts down cell-to-cell communication to show that different kinds of SCN neurons did not have rigidly defined roles

  3. Savannah Meagher- 2nd on September 13th, 2009 7:19 pm

    1. The neurons get out of synch and capriciously quit or start oscillating again.
    2.Individual cells are sloppy and must communicate with one another to establish a conherent 24-hour rhythm, says Herzog.
    3.The SCN includes many kinds of neurons that make different neurochemicals and connections within the SCN and to other parts of the brain.
    4.Webb digested slices of mouse SCN with enzymes to isolate individual neurons and then plated the cells sparsely on a dish.
    5.the cells had been genetically engineerdto glow whenerver they expressed the time -keeping gene Period 2.

  4. Andrew Crisp 1st on September 14th, 2009 4:20 pm

    1.The glow tells the isolated nerve cell is busy keeping time.
    2.The neurons get out of synch and capriciously quit or start oscillating again.
    3.These cells, remarkably, contain the machinery to generate daily, or circadian, rhythms in gene expression and electrical activity.
    4.The cells exposed SCN to the drug TTX, a pufferfish toxin that shuts down cell-to-cell communication.
    5.The SCN includes many kinds of neurons that make different neurochemicals and connections within the SCN and to other parts of the brain.

  5. Andrew Rice on September 14th, 2009 5:20 pm

    Andrew Rice 5th period Sept 14

    1. Alexis Webb studies a nerve cell at washington.
    2.I learned that if the cell glows it is busy keeping time.
    3.Cells that are isolated keep time by themselfs daily.
    4.They are unreliable alone though.
    5.The biological clock adds up to 20,000 neutrons.

  6. TARRELL CLEMONS 1ST PERIOD 9/14/09 on September 14th, 2009 5:39 pm

    The biological clock, a one-square millimeter area of the brain called the suprachiasmic nucleus, or SCN, just above the roof of the mouth and atop the crossing of the optic nerves, comprises about 20,000 neurons.

    These cells, remarkably, contain the machinery to generate daily, or circadian, rhythms in gene expression and electrical activity. But the individual cells are sloppy and must communicate with one another to establish a coherent 24-hour rhythm, says Herzog.
    The glow tells her the isolated nerve cell is busy keeping time.The cells had been genetically engineered to glow whenever they expressed the time-keeping gene Period 2. (The cells came from transgenic mice where the Period 2 gene had been linked to one found in firefly tails.)

    “We’ve known for more than 15 years that unicellular organisms like cyanobacteria can keep 24-hour time, and isolated cells from the marine snail eye can as well,” says Herzog. “But nobody was sure whether individual cells in vertebrates are circadian pacemakers.”

  7. Avery Wilson 4th per. on September 14th, 2009 7:54 pm

    By themselves, nuerons get out of sync.
    Nuerons attach themselves to glass and grow.
    These cells contian the machinery to generate daily rythms.
    The cells were genetically engineered.
    All of the cells can contain a 24 hour rythm.

  8. Marilynlarry6th on Sept. 13, 2009 on September 14th, 2009 8:26 pm

    a girl is found murdered in a school and the teacher is questioned

  9. Sarah Christopherson 5th on September 14th, 2009 9:16 pm

    1 Alexis Webb enters a small room at Washington University in St. Louis with walls.
    2 Floor the ceiling and floors were dark green and blue shutters when he sat down over the microscope.
    3 the black box with drapes she had a little cell that she put on the microscope with the light dim.
    4 she could tell from the glowing cells that they were busy keeping time.
    5 Alexis Webb was a graduate student from neuroscience program.

  10. Elizabeth Sanders 1st period on September 14th, 2009 9:49 pm

    Article Summary

    September 14, 2009

    Individual cells isolated from the biological clock can keep daily time all by themselves.

    The biological clock, a one square millimeter area of the brain called the Suprachiasmic Nucleus or SCN.

    The SCN includes many kinds of neurons.

    The SCN is the master clock that synchronizes other biological clocks, like your liver or your lung.

    Neurons of the SCN make different neurochemicals and connections within the SCN and other parts of the brain.

  11. ADAYZAH watkins 6 on September 15th, 2009 7:23 pm

    1. Alexis Webb research a nerve cell at washington.
    2.I learned that if the cell glows it is busy keeping time.
    3.Cells that are isolated keep time by themselfs daily.
    4.They are unreliable alone though.
    5.The biological clock adds up to 20,000 neutrons

  12. Sam Neighbors on September 15th, 2009 8:28 pm

    1 cells can keep track of time for a short period of time with out comunikating to each other.
    2. but the cells are unreadable
    3.the reseacher say that if you get the cell the nutrents it neads it will roun for months.
    4. the floors and the ceiling were dark green and blue shutters over the microscope
    5.the SCN is the master clock that synchronizes other biological clocks, like your liver or your lungs.

  13. austin xayavong 5th on September 16th, 2009 11:02 pm

    1. i have learned that when its green it keeps busy.
    2. i have learned that it has 20.000 nutrons

  14. Courtney Terry per.4th on August 31,2009 on September 18th, 2009 10:24 pm

    1. The neurons gets out of synch and capriciously quit or start scillating again.
    2. The cell had been genetically engineerdto glow whenever they expressed the time keeping gene period 2.
    3. The biological clock adds up to 20,000 neutrons.
    4. By themselves, nuerons get out of sync.
    5. The SCN included many kinds of neurons.

  15. Taylor Brown (Period 5) on September 20th, 2009 6:57 pm

    Fact 1:Alexis Webb bends over a microscope to find a glowing single cell nerve
    Fact 2:The cells only golw whenever they reach the time keeping gene
    Fact 3:The genes can keep the light for a full 24 hour day but they dont have to
    Fact 4:She fuond that the single cells find it harder to keep a constant rythm
    Fact5:After exposing the cell to the toxins again some of themchanged their behavior
    Opinion:This was a good article that challenged me while giving me some more interesting facts to look forward to.

  16. Brady Purdin (2nd Period) on September 21st, 2009 8:11 am

    September 21, 2009 Article Summary Individual Cells Isolated From Biological Clock Can Keep Daily Time, But Are Unreliable

    1. Nikhil Angelo and James Huettner demonstrated that individual cells isolated from the biological clock can keep daily time all by themselves.
    2. The neurons get out of synch and capriciously quit or start oscillating again.
    3. The biological clock is a one-square millimeter area of the brain called the suprachiasmic nucleus.
    4. SCN contain the machinery to generate daily, or circadian, rhythms in gene expression and electrical activity.
    5. We’ve known for more than 15 years that unicellular organisms like cyanobacteria can keep 24-hour time.

  17. Amari Anderson 1st Period on September 21st, 2009 8:51 am

    1. Individual cells isolated from the biological clock, can keep daily time all by theirselves.
    2.The cells by their selves are not relieble when it comes to tell time.
    3. the SCN includes many kind of nuerons that makes different neurochemicals and connections with SCN and other parts of the brain.
    4. The cells have been genictically engineered when they have the gene to take time.
    5. paradoxily the slopiness of the clock is what makes the cells so precise.

  18. Zach Bennett - 1st Period on September 21st, 2009 4:04 pm

    alex webb went in a room with the walls ,ceiling , and floor painted dark green . and when the lights turned off through the microscope she could she cells. if it glows you know that thatbit is busy. the optic nerves comprises about 20,000 neurons . when the time keeping reaches only then the cell will glow. researches are now focusing on the connection that helps stabilize the oscillitars.

  19. Andrew Crisp 1st on September 21st, 2009 4:24 pm

    1.Alexis Webb sees a glow when bending over a microscope in a black box draped with black cloth.
    2.The glows tells her the islolated nerve cell is bus keeping time.
    3.The biological clock is a one-square millimeter area of the brain called the suprachiasmic nucleus.
    4.The floors and the ceiling were dark green and blue shutters over the microscope.
    5.The biological clock adds up to 20,000 neutrons

  20. Caroline Whorton 6th period on September 21st, 2009 4:27 pm

    When you see a glow under a microscope, it means the isolated nerve cell is busy keeping time.
    Cells from the biological clock can keep daily time all by their selves.
    Individual cells are very unreliable.
    Neurons quit or start oscillating.
    Researchers are working on synchronize and stabilize these oscillators.

  21. Raine Aslan - 4th period on September 21st, 2009 6:03 pm

    1. Individual cells isolated from the biological clock can keep daily time all by themselves.
    2. However, by themselves, they are unreliable.
    3. Individual cells are sloppy and must communicate with one another to establish a conherent 24-hour rhythm.
    4. The SCN is the master clock that synchronizes other biological clocks.
    5. It includes many kinds of neurons that make different neurochemicals and connections within the SCN and to other parts of the brain.

  22. Anthony Pellegrini 1st period on September 21st, 2009 8:16 pm

    1. Through a microscope a person can see a single nerve cell.
    2. The glow in the pictures tells you that the nerve cell is keeping busy.
    3. These cells contain the machinery to genearte daily or circadian, in rythms of gene expression and electrical activity.
    4.Scientists have known for more than 15 years unicelluar organisms like cyanobacteria can keep for 24 hour time.
    5. SCN includes the many kinds of neurons that make different neurochemicals, and connections within the SCN, and to the other parts of the brain.

  23. hunter webber 6th on September 22nd, 2009 6:24 am

    1. Floor the ceiling and floors were dark green and blue shutters when he sat down over the microscope.
    .2. Webb digested slices of mouse SCN with enzymes to isolate individual neurons and then plated the cells sparsely on a dish.
    3. the cells had been genetically engineerdto glow whenerver they expressed the time -keeping gene Period 2.
    4. They arent that reliable and start and stop over time.
    5. i have learned that it has 20.000 nutrons

  24. Kris Levasseur 2nd period on September 26th, 2009 9:02 am

    1. Alexis Webb enters a small room at Washington University in St. Louis turns off the lights and bends over a microscope in a black box draped with black cloth
    2.The glow tells her the isolated nerve cell is busy keeping time.
    3. The neurons, however, by themselves, they are unreliable.
    4.The neurons get out of synch and capriciously quit or start oscillating again.
    5.The biological clock, a one-square millimeter area of the brain called the suprachiasmic nucleus.

  25. Dylan Clark 4th period on September 27th, 2009 8:40 pm

    1) Alexis Webb studies nerve cells under a microscope at Washington University.
    2) Webb was a graduate in the neuroscience program.
    3) The biologly clock, a one sqaure millimeter area of the brain called the suprachiasmic nucleus, comprises of about 20,000 neurons.
    4) These cells have the machinery to generate every day, or circadion, rhythms in gene expression and electrical activity.
    5) These features make the suprachiasmic nucleus a flexible clock that can reset to stay in synch in an ever-changing environment.

  26. claire lorino 4th on September 27th, 2009 10:50 pm

    Alexis Webb sees a glow when bending over a microscope in a black box draped with black cloth. A person can see a single nerve cell through a microscope.The glows tells her the islolated nerve cell is bus keeping time.The biological clock is a one-square millimeter area of the brain called the suprachiasmic nucleus.Cells from the biological clock can keep daily time all by their selves.Individual cells are very unreliable. Researchers will be focusing on connections that help synchronize and stabilize these biological oscillators.

    .

  27. Drake Horner 1st Period on September 28th, 2009 2:32 pm

    1.Alexis Webb studies Nerve Cells
    2.Induvidual Cells alone are unreliable
    3.Genes can keep light for a full day
    4.The cells were Engineered Geneaticly
    5.The biological clock adds up to 20,000 neutrons

  28. hunter walker 1 on September 28th, 2009 4:30 pm

    Alexis Webb sees a glow when bending over a microscope in a black box draped with black cloth
    Webb was a graduate in the neuroscience program
    These features make the suprachiasmic nucleus a flexible clock that can reset to stay in synch in an ever-changing
    The neurons get out of synch and capriciously quit or start oscillating again
    They arent that reliable and start and stop over time

  29. Will Cook 4th period on September 28th, 2009 4:42 pm

    9-27-09

    Alexis Webb sees a glowing object when bending over the microscope that was in a black box draped with black cloth. She knows that the glows mean the isolated nerve cell is keeping time. It is a one-square millimeter part of the brain called a suprachiasmic neucleus. It comprises about 20,000 neurons. The biological clock can stay in synch in an ever-changing environment.

  30. Elijah Hurskin 4th period 9/27/09 10:00 on September 28th, 2009 10:00 pm

    1) Alexis Webb enters a small room at Washington University in St. Louis turns off the lights and bends over a microscope in a black box draped with black cloth
    2) Webb was a graduate in the neuroscience program.
    3) Webb digested slices of mouse SCN with enzymes to isolate individual neurons and then plated the cells sparsely on a dish.
    4)The neurons get out of synch and capriciously quit or start oscillating again.
    5)The biological clock, a one-square millimeter area of the brain called the suprachiasmic nucleus

  31. Elijah Hurskin 4th period 9/27/09 10:00 on September 28th, 2009 10:00 pm

    1) Alexis Webb enters a small room at Washington University in St. Louis turns off the lights and bends over a microscope in a black box draped with black cloth
    2) Webb was a graduate in the neuroscience program.
    3) Webb digested slices of mouse SCN with enzymes to isolate individual neurons and then plated the cells sparsely on a dish.
    4)The neurons get out of synch and capriciously quit or start oscillating again.
    5)The biological clock, a one-square millimeter area of the brain called the suprachiasmic nucleus.

  32. Marilynlarry Sept. 29 on September 29th, 2009 8:12 am

    1 Single cells sometimes will be very robust and rhythmic.
    2 Neurons will actually attach to the glass and grow.
    3 As long as you give them all of the nutrients they need, they’ll live for months.
    4 Neurons get out of synch and capriciously quit or start oscillating again.
    5 Through a microscope a person can see a single nerve cell.

  33. Ruhi singh 2nd period on October 1st, 2009 5:35 pm

    Article
    1.Alexis Webb enters a small room sees a single nerve cell
    on a glass cover slip glowing dimly.
    2.The glow of the isolated nerve cell is busy keeping time.
    3 .Individual cells isolated from the biological clock can keep daily time all by themselves.
    4.Genes can keep light for a full day
    5.The biological clock adds up to 20,000 neutrons.

  34. Kathryn Walls 6th period science on October 12th, 2009 10:57 am

    (1)Alex Webb sees a glow when bending over a microscope.(2) Genes Can keep light for a full day. (3) Individual cells alone are unreiliable.(4) The cells were engenered geneticly.(5) The bilogical clock adds up 20,000 neutrons.

  35. hunter walker 1 on October 12th, 2009 6:48 pm

    1. single cells sometimes will be very robust and or rhythmic.
    2. webb was a graduate in nueroscience program.
    3. genes can keep light for a whole day.
    4. the cells were genetically engineered.
    5. through a microscope a person may see a single cell nerve.

  36. Joshua Sanchez-6 on October 12th, 2009 9:41 pm

    1. Alexis found nerve cells that where glowing in Washington.
    2. Isolated cells can keep time on their own when they are glowing.
    3. When they are alown then they cabt keep time.
    4.The SCN can adjust to the time and its enviroment.
    5. The biological clock adds up to 20,000 neurons.

  37. connor ferguson 1st period on November 2nd, 2009 9:57 pm

    1.The glow tells here the cell is keeping time.
    2.The neurons get out of synch and capriciously quit or start oscillating again.
    3.A biological clock a one-square millimeter area of the brain compromises of 20000 neurons.
    4.The cells are sloppy and must communicate with one another to establish a 24-hour rhythm.
    5.The researchers are now focusing on the connections that help synchronize and stabilize these biological oscillators.

  38. Tara Harville- 1st per. on November 8th, 2009 8:53 pm

    Alexis Webb enters a small room at Washington University in St. Louis with walls, floor and ceiling painted dark green, shuts the door, turns off the lights and bends over a microscope in a black box draped with black cloth.
    The glow tells her the isolated nerve cell is busy keeping time.
    Through the microscope, she can see a single nerve cell on a glass cover slip glowing dimly.
    The neurons get out of synch and capriciously quit or start oscillating again.
    These cells, remarkably, contain the machinery to generate daily, or circadian, rhythms in gene expression and electrical activity.

  39. Derrell Sledge 1st period on November 9th, 2009 6:52 pm

    1. Cells can keep track of time for a short period of time with out comunikating to each other.
    2. but the cells are unreadable
    3.the reseacher say that if you get the cell the nutrents it neads it will roun for months.
    4. the floors and the ceiling were dark green and blue shutters over the microscope
    5.the SCN is the master clock that synchronizes other biological clocks, like your liver or your lungs

  40. Matthew Eppard 1st Period on November 10th, 2009 8:36 am

    1. The neurons get out of synch and capriciously quit or start oscillating again.
    2.Individual cells are sloppy and must communicate with one another to establish a conherent 24-hour rhythm, says Herzog.
    3.The SCN includes many kinds of neurons that make different neurochemicals and connections within the SCN and to other parts of the brain.
    4.Webb digested slices of mouse SCN with enzymes to isolate individual neurons and then plated the cells sparsely on a dish.
    5.The cells had been genetically enginered to glow whenerver they expressed the time -keeping gene Period 2

  41. Kelsey schronce 5th perod on November 14th, 2009 2:12 pm

    1. The neurons get out of synch and capriciously quit or start oscillating again.
    2. Isolated cells can keep time on their own when they are glowing.
    3. When they are alown then they cabt keep time.
    4.The SCN can adjust to the time and its enviroment.
    5. The biological clock adds up to 20,000 neurons.

  42. austin xayavong 5th on November 14th, 2009 9:40 pm

    1.Alexis Webb enters a small room at Washington University in St. Louis with walls, floor and ceiling painted dark green, shuts the door, turns off the lights and bends over a microscope in a black box draped with black cloth.
    2.The glow tells her the isolated nerve cell is busy keeping time.

  43. hunter walker-1 on November 16th, 2009 4:39 pm

    1. the cells are unreadable.
    2. genes can keep the light for a full day.
    3. alexis webb studies nerve cells.
    4. the cells can keep track of time.
    5. single cells sometimes will be very robust and rhythmic.

  44. Stephen Doran 2nd on November 16th, 2009 6:35 pm

    1.20,000 neurons
    2.isolated cells can keep time on their own when they are glowing
    3.the cells have been genetically indangerded

  45. deondre surles 6th period on December 7th, 2009 5:23 pm

    The cells have been genectically indangered.

    The cells keep the time.

    The biological counts up to 20,000.

    Genes can keep light for a whole day.

  46. Robert McClintock on December 7th, 2009 6:08 pm

    I heard that Alex saw the nerve cell glowing dimly while keeping time. A one-square mm area of the brian called the Suprachiamic Nuclues, or SCN. The cell contains machinery to generate daily, or circadian, rhythms in gene expression and electrical activity. SCN includes many kinds of neurons that make different neurochemicals and connections within the nevous system. I learned that the neurons will actually attach to the glass and grow.

  47. alex 4th period on December 14th, 2009 7:24 pm

    Alex Webb studies nerve cells under microscopes at the Washington University.
    2 The biologly clock, a one sqaure millimeter area of the brain called the suprachiasmic nucleus, has about 20,000 neurons.,
    3 he was a graduate in the neuroscience program.
    4 That makes the nucleus a good, flexible clock that can reset to stay in synch in a changing environment.
    5 The cells have the ability to generate every day and make electrical activity.

  48. alex 4th period on December 14th, 2009 7:25 pm

    1 Alex Webb studies nerve cells under microscopes at the Washington University.
    2 The biologly clock, a one sqaure millimeter area of the brain called the suprachiasmic nucleus, has about 20,000 neurons.,
    3 he was a graduate in the neuroscience program.
    4 That makes the nucleus a good, flexible clock that can reset to stay in synch in a changing environment.
    5 The cells have the ability to generate every day and make electrical activity.

  49. Kaylee Thom 4th on December 14th, 2009 11:00 pm

    The glow in the nerve is the nerve keeping time.
    The neurons without each other get out of synch.
    The individual cells can keep daily time all by themselves.
    The biological clock is one square millimeter of the brain.
    The SCN lots of neurons that make neurochemicals and connections within the SCN and to other parts of the brain.

  50. Michael worshim -6th on December 15th, 2009 3:08 pm

    Alexis webb is from washington Unversity
    Alexis Webb gradutaed in the program of neuroscience
    Glow tells her the isolated nerve cell is keeping time
    The isolated cells can keep time all by thereselves
    neruons by themsevles are unreliable.

  51. mauhaun taheri 1st on January 18th, 2010 2:56 pm

    Cells have magnifictly indangered.
    Isolated variables keep time by themselves.
    A clock is one square centimeter of the brain.
    One centimeter has 20,000 nuerons.
    Cells generate everyday.

  52. Cassidy McCarter-4th on January 25th, 2010 4:42 pm

    1. The neurons get out of synch and capriciously quit or start oscillating again.
    2.Individual cells are sloppy and must communicate with one another to establish a conherent 24-hour rhythm, says Herzog.
    3.The SCN includes many kinds of neurons that make different neurochemicals and connections within the SCN and to other parts of the brain.
    4.Webb digested slices of mouse SCN with enzymes to isolate individual neurons and then plated the cells sparsely on a dish.
    5.the cells had been genetically engineerdto glow whenerver they expressed the time -keeping gene Period 2.

  53. Scott Urban 5th period on January 25th, 2010 7:24 pm

    1.20,000 neurons

    2.single cells sometimes will be very robust and or rhythmic

    3.Nikhil Angelo and James Huettner demonstrated that individual cells isolated from the biological clock can keep daily time all by themselves

    4.the biological clock a one-square millimeter area of the brain called the SCN, just above the roof of the mouth and atop the crossing of the optic nerves

    5.Genes can keep light for a whole day

  54. KELSEY SCHRONCE 5th PERIOD on February 1st, 2010 9:36 pm

    .The glow tells the isolated nerve cell is busy keeping time.
    2.The neurons get out of synch and capriciously quit or start oscillating again.
    3.These cells, remarkably, contain the machinery to generate daily, or circadian, rhythms in gene expression and electrical activity.
    4.The cells exposed SCN to the drug TTX, a pufferfish toxin that shuts down cell-to-cell communication.
    5.The SCN includes many kinds of neurons that make different neurochemicals and connections within the SCN and to other parts of the brain.

  55. Reagan Locke 4th on February 7th, 2010 10:23 pm

    The neurons gets out of sync and capriciously quit or start scillating again. Cells have magnificently endangered.The biological clock can stay in sync in an ever-changing environment.Webb digested slices of mouse SCN with enzymes to isolate individual neurons and then plated the cells sparsely on a dish. A person can see a single nerve cell through a microscope.

  56. deondre surles 6th period on March 8th, 2010 5:01 pm

    The woman can see a nerve cell growing.

    The nerve cell is keeping time.

    The neurons are unreliable.

    They aren’t reliable when it comes to telling time.

  57. Elijah Hurskin 4th period 3/8/10 6:14 pm on March 8th, 2010 7:14 pm

    1)Alexis Webb a graduate student in the neuroscience program has demonstrated that individual cells isolated from the biological clock can keep daily time all by themselves.
    2) She is not the only one to come up with this
    3)The biological clock, a one-square millimeter area of the brain called the suprachiasmic nucleus, or SCN, just above the roof of the mouth and atop the crossing of the optic nerves, comprises about 20,000 neurons.
    4)These cells, remarkably, contain the machinery to generate daily, or circadian, rhythms in gene expression and electrical activity.
    5)But the individual cells are sloppy and must communicate with one another to establish a coherent 24-hour rhythm, says Herzog.

  58. Kelsey schronce 5th perod on March 9th, 2010 11:49 am

    ~ Alexis Webb sees a glow when bending over a microscope in a black box draped with black cloth
    ~ The glows tells her the islolated nerve cell is bus keeping time
    ~ The biological clock just above the roof of the mouth and atop the crossing of the optic nerves, comprises about 20,000 neurons
    ~ These cells contain the machinery to generate daily or circadian rhythms in gene expression and electrical activity
    ~ But the individual cells are sloppy and must communicate with one another to establish a coherent 24-hour rhythm; these features make the SCN a flexible clock that can reset to stay in synch in an ever-changing environment
    ~ Webb exposed SCN to the drug TTX, a pufferfish toxin that shuts down cell-to-cell communication to show that different kinds of SCN neurons did not have rigidly defined roles

  59. Pamela Hussey-6th Period on March 9th, 2010 7:36 pm

    1. Alexis Webb studies nerve cells under a microscope at Washington University.
    2. Isolated cells can keep time on their own when they glow.
    3. Cells are unreadable.
    4. The neurons by themselves, are unreliable.
    5. Some scientists felt that all of the cells in the SCN would be intrinsically rhythmic.

  60. brett stoddard 5th period on March 29th, 2010 7:39 pm

    !).Alexis Webb is a girl
    2).Glow tells her that the nerve cell is busy keeping time.
    3).Alex Webb is a graduate student in the neuroscience program.
    4). The suprachiasmic nucleus contains about 20,000 neurons.
    5). The suprachiasmic nucleus is known as the SCN.

  61. Max Stephenson 2nd on April 16th, 2010 11:16 am

    The SCN lots of neurons that make neurochemicals and connections within the SCN and to other parts of the brain.The biologly clock, a one sqaure millimeter area of the brain called the suprachiasmic nucleus, has about 20,000 neurons.,he was a graduate in the neuroscience program.Individual cells isolated from the biological clock can keep daily time all by themselves.

  62. Chase R. Massey 5th Period on May 3rd, 2010 7:41 am

    1) Cells are unreadable.
    2) The neurons by themselves are unreliable.
    3) 1 centimeter has 20,000 neurons
    4) A clock is one centimeter of the brain.
    5) The cells can keep track of time.

  63. Devin Platts 1st on May 10th, 2010 9:54 am

    1. nerve cells can

  64. Devin Platts 1st on May 10th, 2010 10:00 am

    1.The neurons get out and capriciously start oscillating again.
    2. The glow of the neurons shows you that it is working.
    3.Alexis Webb walked into Washingt5on college and looked in the microscope and saw a glowing nueron and thought it was weird.
    4.The scientist are focused on the connecvtions

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